Secondary Antibodies
Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies
(44,180)
Mouse Secondary Antibodies
(14,490)
Rabbit Secondary Antibodies
(6,301)
Donkey Secondary Antibodies
(4,319)
Rat Secondary Antibodies
(1,234)
Sheep Secondary Antibodies
(701)
Chicken Secondary Antibodies
(403)
Bovine Secondary Antibodies
(257)
Pig Secondary Antibodies
(76)
Horse Secondary Antibodies
(74)
Alpaca Secondary Antibodies
(62)
Hamster Secondary Antibodies
(57)
Biosimilar Secondary Antibodies
(9)
Human Secondary Antibodies
(7)
Duck Secondary Antibodies
(3)
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Goat anti-Human Kappa Light Chain Secondary Antibody (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunoassay,Immunodiffusion,Immunohistochemistry,Immunoprecipitation,Western Blot |
| Concentration | 4.5mg/mL |
| Antigen | Kappa Light Chain |
| Gene Symbols | IGKC |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Immunoassay |
| Gene Alias | HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809 |
| Gene ID (Entrez) | 3514 |
| Classification | Polyclonal |
| Immunogen | Purified Human Kappa Chain |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with kappa and light chains on all human immunoglobulins. This antibody has been pre-adsorbed against mouse serum proteins. |
Goat F(ab')2 anti-Human IgM Heavy Chain Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgM |
| Concentration | LYOPH |
| Antigen | IgM Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin |
| Gene Alias | Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain |
| Gene ID (Entrez) | 3507 |
| Classification | Polyclonal |
| Immunogen | Purified Human IgM |
| Reconstitution | Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy mu chains on human IgM. This antibody has been pre-adsorbed against human IgA + IgG. |
Goat anti-Chicken IgG (H+L) Secondary Antibody, DyLight 680 (Pre-adsorbed), Novus Biologicals™
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Chicken |
| Host Species | Goat |
| Conjugate | DyLight 680 |
| Applications | Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000 |
| Classification | Polyclonal |
| Immunogen | Chicken IgG whole molecule |
| Reconstitution | Reconstitute with 100 ul deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins. |
Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Donkey Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Sheep |
| Host Species | Donkey |
| Conjugate | Unconjugated |
| Applications | ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence, Fluorophore-linked immunosorbent assay |
| Classification | Polyclonal |
| Immunogen | Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Sheep IgG whole molecule in donkey. |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins. |
Goat anti-Hamster IgG (H+L) Secondary Antibody, DyLight 680 (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Hamster |
| Host Species | Goat |
| Conjugate | DyLight 680 |
| Applications | Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:10000 |
| Classification | Polyclonal |
| Immunogen | Armenian and Golden Syrian Hamster IgG, whole molecule |
| Reconstitution | Reconstitute with 100 ul deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Mouse and Rat Serum Proteins. No reaction was observed against Mouse or Rat Serum Proteins. This antibody will react with heavy chains of Golden Syrian & Armenian Hamster IgG and with light chains of most Golden Syrian & Armenian Hamster immunoglobulins. |
Goat anti-Mouse IgG Fc Secondary Antibody, DyLight 680, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Mouse |
| Host Species | Goat |
| Conjugate | DyLight 680 |
| Applications | Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000 |
| Classification | Polyclonal |
| Immunogen | Goat anti-Mouse IgG Fc fragment Secondary Antibody was produced by repeated immunization with Mouse IgG F(c) fragment in goat. |
| Reconstitution | Reconstitute with 100 ul deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Mouse IgG F(ab) This antibody will react with heavy chains of Mouse IgG. Minimal reactivity is expected against other Mouse immunoglobulins. |
Goat anti-Rat IgM Heavy Chain Secondary Antibody, Biotin, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Rat |
| Host Species | Goat |
| Conjugate | Biotin |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgM Heavy Chain |
| Gene Symbols | IGHM |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000, Immunohistochemistry-Paraffin 1:1000 - 1:5000 |
| Gene Alias | Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain |
| Gene ID (Entrez) | 3507 |
| Classification | Polyclonal |
| Immunogen | Rat IgM u chain |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against other rat heavy or light chain proteins. |
Goat anti-Monkey IgM Heavy Chain Secondary Antibody, FITC, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C in the dark. |
|---|---|
| Target Species | Monkey |
| Host Species | Goat |
| Conjugate | Fluorescein |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgM Heavy Chain |
| Gene Symbols | IGHM |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Gene Alias | Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain |
| Gene ID (Entrez) | 3507 |
| Classification | Polyclonal |
| Immunogen | Monkey IgM u (mu) chain |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against other Monkey heavy or light chain proteins. |
Donkey anti-Rabbit IgG Fc Secondary Antibody, Novus Biologicals™
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Donkey Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Rabbit |
| Host Species | Donkey |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:2000 - 1:20000, ELISA 1:100000, Immunohistochemistry 1:1000 - 1:5000 |
| Classification | Polyclonal |
| Immunogen | Rabbit IgG F(c) fragment |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Rabbit IgG F(ab). |
Donkey anti-Sheep IgG (H+L) Secondary Antibody, FITC (Pre-adsorbed), Novus Biologicals™
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Donkey Polyclonal Antibody
| Content And Storage | Store at 4C in the dark. |
|---|---|
| Target Species | Sheep |
| Host Species | Donkey |
| Conjugate | Fluorescein |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Sheep IgG whole molecule |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbits and Rat Serum Proteins. |
Goat anti-Golden Syrian Hamster IgG (H+L) Secondary Antibody, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Golden Syrian Hamster |
| Host Species | Goat |
| Conjugate | Unconjugated |
| Applications | Dot Blot,ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 2.1 mg/ml |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:2000 - 1:10000, ELISA 1:25000 - 1:125000, Immunohistochemistry 1:1000 - 1:5000, Dot Blot |
| Classification | Polyclonal |
| Immunogen | Golden Syrian Hamster IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. |
Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
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Donkey Polyclonal Antibody has been used in 3 publications
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Rabbit |
| Host Species | Donkey |
| Conjugate | Unconjugated |
| Applications | ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunodiffusion,Immunohistochemistry,Immunohistochemistry (Frozen),Immunoprecipitation,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 4.5mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Western Blot 1:100-1:2000, Flow Cytometry, ELISA 1:100-1:2000, Immunohistochemistry 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunodiffusion, Immunoassay |
| Classification | Polyclonal |
| Immunogen | Purified Rabbit IgG (H&L) |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody has been cross-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep. |
Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, DyLight 633, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage. |
|---|---|
| Target Species | Guinea Pig |
| Host Species | Goat |
| Conjugate | DyLight 633 |
| Applications | Flow Cytometry,Immunomicroscopy |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000 |
| Classification | Polyclonal |
| Immunogen | Purified Guinea Pig IgG (H&L) |
| Reconstitution | Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on guinea pig IgG and light chains on all guinea pig immunoglobulins |
Goat F(ab')2 anti-Human IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin |
| Classification | Polyclonal |
| Immunogen | Purified Human IgG (H&L) |
| Reconstitution | Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on human IgG and light chains on all human immunoglobulins. This antibody has been pre-adsorbed against bovine, mouse, and rabbit serum proteins. |
Goat anti-Human IgG Fc Secondary Antibody, Biotin (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | Biotin |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Gene Accession No. | O75015, O75015, O75015 |
| Concentration | 1.5mg/mL |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Western Blot 1:2000 - 1:20000, ELISA 1:2000 - 1:20000, Immunohistochemistry 1:500 - 1:5000, Immunohistochemistry-Paraffin |
| Classification | Polyclonal |
| Immunogen | Purified Human IgG, Fc fragment |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on human IgG. This antibody has been pre-adsorbed against human IgA + IgM, bovine, horse, mouse, and rabbit serum proteins. |